CytoFLEX Features Page

Quality performance, multiple configurations for real power on your benchtop

The high-performance CytoFLEX system offers scalable detection capabilities for up-to three lasers and 13-color research flow cytometry right on your benchtop.

Ready to run in minutes, not hours with CytoFLEX

CytoFLEX Flow Cytometer System


The CytoFLEX system is easy to learn, easy to operate and easy to maintain.

  • Superior sensitivity and resolution for excellent fluorescence and nanoparticle detection
  • Compact, reliable design fits right on your benchtop (<50lbs, 16”x16”x13”)
  • Future-proof system with easy upgrade options
  • Powerful performance with up-to 21 flexible configurations (up-to three Lasers, 13 colors)
  • CytExpert experiment-based software for easy multicolor setup and QC
  • Versatile plug-n-play filters (bandpass-only light collection)
  • Integrated optics for alignment stability
  • Accommodates 12x75mm and microtiter tubes


An intelligent patent-pending optical design optimizes excitation from up-to three lasers (488nm; 638nm; 405nm) and light collection efficiency. With unique flow cell design and integrated optics, the innovative Wavelength Division Multiplexing (WDM) detection module includes solid-state, high efficiency, low-noise detectors for excellent performance. The WDM’s bandpass-only design guarantees flexibility with easily changeable filters and 4-13 configurations.

8-peak Rainbow Beads


Nano particle detection

When equipped with a violet laser, the CytoFLEX can be set up to measure Side Scatter from 405nm for enhanced nanoparticle detection. Mie theory predicts that the scattering cross section of a particle, and thus its scattering intensity, depends on the wavelength of light, the angle of collection, and the size, shape, and refractive index of the particle. All other factors being equal, using a shorter illumination wavelength will result in an increase in scattering cross section, and thus more scattered light. The CytoFLEX offers light scattering parameter measurements from the 488nm laser light, and optionally from the 405nm laser to enhance small particle detection.

CytoFLEX Nanoparticle Detection 

Maximize investment in CytoFLEX with custom configuration options

With future-proof upgrade options for up-to 13 colors, the CytoFLEX system evolves as your application needs change. Upgrade your configuration in less than one day knowing that all CytoFLEX instruments have been fully tested across all configuration types.


Excitation Fluorescence
488nm 525/40 BP FITC, Alexa Fluor 488, CFSE, Fluo-3
585/42 BP PE, PI
610/20 BP ECD, PE-Texas Red, PE-CF594, PI
690/50 BP PC5, PC5.5, PerCP, PerCP-Cy5.5, PI
780/60 BP PC7
638nm 660/10 BP APC, Alex Fluor 647, eFluor 660
712/25 BP APC-Alexa Fluor 700, Alexa Fluor 700
780/60 BP APC-Alexa Fluor 750, APC-Cy7, APC-H7, APC-eFlour 780
405nm 450/45 BP Pacific Blue, V450, eFluor 450, BV421
525/40 BP Krome Orange, AmCyan, V500, BV510
610/20 BP Violet610, BV605, Qdot 605
660/10 BP Violet660, BV650, Qdot 655
780/60 BP Viole780, BV785, Qdot 800