Cytomics FC500 Flow Cytometer

The Cytomics FC 500 Flow Cytometer Automated tube-based acquisition for clinical assays. It has the capacity to conduct 5-color analysis using either a single 488 nm (blue) laser or a dual 488 nm/635 nm (blue/red) laser. The FC500 is available with a carousel tube loader or a Multi-Platform Loader (MPL) that accommodates both tubes and plates.

  • stemCXP Software for hematopoietic stem cell enumeration
  • tetraCXP Software for TBNK analysis
  • ADC technology for color compensation
  • Compensate during acquisition and in listmode analysis

Choose a Cytomics FC500 Flow Cytometer Model

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Cytomics FC500 Flow Cytometer Features

System Configurations

  • 5 color analysis using a single blue (488 nm) laser.
  • Optional red (635 nm) laser available
  • Optional plate-based analysis via optional Multi-platform loader (MPL)


  • Streamlined data entry and reporting with bi-directional LIS connectivity when used with Data Innovations Instrument Manager 8.05-8.12
  • QC functionality with Levey-Jennings reports
  • 21CFR Part 11 support via MXP software

Set up and aquisition

Set up and Acquisition 

  • Vortex of individual tube prior to acquisition
  • Two forward scatter settings for effective sizing
  • Flow Check and Flow Set Fluorospheres for fast automated set up
  • IQAP Quality Assurance Program to compare performance
  • Barcode identification of carousel number, position number and tube label
  • 0.5 µm particle resolution

Clinical Assays

  • Ready to use, pre-optimized reagents make sample prep easier than ever
  • Improve lab safety with reduced sample handling with no wash protocol
  • Process controls including low level CD4 counts
  • Built-in reliability and intra-panel checks
  • Sample flagging with insufficient T cells

Cytomics FC500 Flow Cytometer Specifications

Detection Photodiodes (forward & side scatter), Photomultiplier tubes (fluorescence)
Resolution 0.5 μm diameter particles. HPCV is <2% to <4% depending on the laser.
Performance <300 MESF for PE, <600 MESF for APC, <600 MESF for FITC
Flow Cell 150 x 450 µm rectangular channel BioSense enhanced optics, Quartz mounted with vertical flow path for superior hydrodynamic focusing
Signal Processing Digital System with 4 decade data display
Power Requirements 50/60 Hz, 100-240 VAC
Item Specifications Referenced 626553

Cytomics FC500 Flow Cytometer Documentation

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