Balancing Your Rotor
- A rotor must never be run with buckets missing, although opposing buckets may be left empty.
- All opposing loads must balance within a certain weight as specified by the centrifuge manufacturer’s instruction manual.
- If opposing buckets are run with a partial load of tubes in their adapters, these tubes must be arranged symmetrically, both with respect to the pivotal axis of each bucket and across the center of rotation. With some partial loads, it may be difficult or impossible to achieve the correct symmetry in both sets of opposing buckets. The simplest solution is to fill one or more tubes of the same size with water, or a denser liquid if necessary, and use them to balance the load symmetrically.
Correct Rotor Symmetry
Most centrifuges are equipped with an imbalance detector which turns the centrifuge off before any eccentric rotation caused by a load imbalance can damage the drive shaft or bearings. However, the improper distribution of tubes in carriers or adapters can cause poor separations even if the imbalance isn’t severe enough to trigger this detector. In these situations, the buckets won’t pivot to the required horizontal position during the run, resulting in poor density separations or re-mixing of sedimented material during deceleration. Also, the possibility of tube breakage during the run is greatly increased when the buckets are not horizontal at operating speed.
You may notice that the centrifuge vibrates when the rotor is accelerating or decelerating at low speeds. This is normal, and occurs as the rotor passes through a so called critical speed range where any small vibrations are temporarily amplified. Your separations will not be disturbed during deceleration, because the centrifugal force is still high enough to stabilize them. However, you should not select an operating speed within the range where these exaggerated vibrations occur. Your instruction manual will tell you what speeds to avoid.
Correct Symmetry When Balancing a Partial Load – A Top View of a Horizontal Rotor
Example of a balanced load:
Assuming all tubes have been filled with an equal amount of liquid, this rotor load is balanced. The opposing bucket sets A-C and B-D are loaded with an equal number of tubes and are balanced across the center of rotation. Each bucket is also balanced with respect to its pivotal axis.
Example of an unbalanced load:
Even if all the tubes are filled equally, this rotor is improperly loaded. None of the bucket loads are balanced with respect to their pivotal axes. At operating speed, buckets A and C will not reach the horizontal position. Buckets B and D will pivot past the horizontal. Also note that the tube arrangement in the opposing buckets B and D is not symmetrical across the center of rotation.