How do I stimulate my cells to induce cytokine expression?

Человек открывает коробку с логотипом Beckman Coulter
There are many ways to achieve cellular stimulation, specific and unspecific. Incubation with a mixture of phorbol myristate acetate and Ionomycin (PMA+I), together with Brefeldin A to inhibit cellular secretion, is widely used as a positive control method.