The PerFix-nc Kit (no centrifuge assay Kit) has been developed to enhance the signal-to-noise ratio of intracellular staining and simplify the workload necessary for the sample preparation. Accurate detection of both intracellular and extracellular epitopes are obtained, while:
- There are no washing steps through the whole STRAIGHT procedure (only a final wash step is described as optional).
- Several surface markers can be added together with the intracellular markers and incubated simultaneously during the permeabilization step.
- Total duration of the procedure and total workload are similar to current procedures for surface staining (e.g. VersaLyse + Fix No Wash), and much shorter than common permeabilization procedures.
- Potential automation of the PerFix-nc STRAIGHT procedure is rendered possible thanks to the removal of the washing steps.