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Joshua Robinson covers the utility of fractionation in the investigation of complex mixtures and polydisperse samples as well as versatility of analytical ultracentrifugation (AUC) for colloid chemistry. He quickly reviews analytical ultracentrifugation (AUC) theory, then discusses deconstructing nano-constructs and how AUC has been used in recent literature to really probe the underlying structure of nano-constructs, nanomaterials. Other topics covered include nanoparticle sample preparation, as well as Beckman Coulter's research and future perspectives.