CytoFLEX Flow Cytometer

The CytoFLEX Flow Cytometer, the first introduction to the CytoFLEX Platform, provides the performance you need in an easy to use system allowing you to focus on the science, not the instrumentation. Its superior sensitivity and resolution throughout all configurations give it the edge over other cytometry systems four times its size. All instruments in the platform utilize CytExpert for CytoFLEX Acquisition and Analysis Software.

CytoFLEX Flow Cytometer Features

Exquisite Sensitivity

Platform Versatility

Low Maintenance

Automation Enabled

  • Optional 96-well plate sample loader module
  • API (Application Programming Interface) allows external software to control the instrument
  • For automated sample processing and data acquisition integrate with the Biomek i-Series Instrument

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CytoFLEX Flow Cytometer Specifications

Detection Photodiodes
Resolution <300 nm particles (488 nm side scatter), <80 nm polystyrene particles (405 nm side scatter), rCV 3%
Performance <30 MESF for FITC, <10 MESF for PE
Flow Cell Patent-pending, alignment-free integrated optics quartz flow cell (420 μm x 180 μm ID) design with >1.3 numerical aperture
Signal Processing Digital system with 7-decade display
Power Requirements 100-240 VAC, 50/60 Hz
Item Specifications Referenced B53000

Content and Resources

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Flow-cytometry-application-note-13-color-panel-t-cell-subsets-whole-blood Contains a comprehensive overview of T cell biology and the implications for multicolor immunophenotyping panel design. Includes a detailed gating strategy for deep immunophenotyping of T cell populations including rare cell types. Also includes step-by-step instructions for setting up the CytoFLEX for multicolor immunophenotyping including use of the Compensation Library.
Flow-cytometry-application-note-8-color-panel-unlysed-whole-blood This whole blood assay does not involve harsh lysis or centrifugation steps, minimizing any cell perturbations from sample handling. This 8-color panel provides a broad survey of peripheral blood leukocytes.
Flow-cytometry-application-note-natural-killer-cells-preeclampsia-model A special population of natural killer cells (uNK) has been implicated in preeclampsia. uNK cells have contact with the trophoblast, modify uterine arteries to promote blood flow, and modulate trophoblast invasion in a dose-dependent manner. In this application note a detailed method for preparing resident NK cells from tissue and an assay to access NK cell activation is provided.
Flow-cytometry-application-note-phagocytosis-of-bacteria-whole-blood Describes an opsonization assay which can be used to assess the phagocytic capabilities of immune cell in whole blood. The benefits of using multicolor flow cytometry for the assay is that different phagocytic potential across various immune cell types can be determined in the same sample.
Flow-cytometry-application-note-dna-binding-dyes-for-viability-assesment Compares viability staining using DRAQ7, DAPI, and 7-AAD in a multicolor immunophenotyping panel. Attributes of each dye are evaluated to aid in selection during panel design.

Technical Documents