RNAdvance Tissue Performance and Data

Agencourt RNAdvance Tissue

Using patented SPRI (Solid Phase Reversible Immobilization) technology, the RNAdvance Tissue kit can extract total RNA from a wide variety of tissues without the hazards and waste removal issues of organic solvents or the time- and labor-consuming steps of vacuum filtration and centrifugation procedures. For most tissues, this extraction process produces higher recovery of total RNA than traditional column-based approaches.

  • Compatible with downstream gene expression analysis techniques:
    • NGS
    • qRT-PCR
    • Microarray
  • Extraction and purification of high-quality RNA from many tissue types
  • Efficient removal of genomic DNA and other contaminants

 

Superior Recovery of RNA from a Variety of Tissue Types Without Sacrificing Purity

RNA was extracted from 10 mg of brain, liver, lung, kidney or tail tissues using the RNAdvance Tissue and other commercially available kits.

 
RNAdvance - RNA Extraction from Tissue - Yield RNAdvance - RNA Extraction from Tissue - Purity

 

(Left) RNA yield was quantified using the NanoDrop (Thermo Fisher Scientific). The RNAdvance Tissue kit recovered higher amounts of RNA from the five different tissue types used than the other commercially available kits.

(Right) Samples were assessed for purity using the NanoDrop (Thermo Fisher Scientific). For all types of tissue, RNAdvance Tissue kit purified RNA with A260/280 ratios within satisfactory ratios.

 

RNAdvance Workflow

RNAdvance Workflow

  1. Lyse tissue in Lysis Buffer and proteinase K
  2. Bind RNA to magnetic beads
  3. Separate magnetic beads from contaminants
  4. Wash magnetic beads with Wash Buffer and 70% ethanol to remove contaminants
  5. Treat samples with DNase I
  6. Rebind RNA to magnetic beads with wash buffer
  7. Wash magnetic beads with 70% ethanol to remove contaminants
  8. Elute RNA from magnetic beads
  9. Transfer to new plate

 

Automation vs. Manual Timing

Estimated hands-on time and total time in hours required to perform 48 and 96 RNAdvance Tissue RNA extractions. Extractions can be performed either manually or automated on a Biomek Workstation. The table represents automation times performed on a Biomek i5 Multichannel. Difference in time between manual and automation is indicated. NR=Not Recommended.

 

RNAdvance Tissue Manual Automated
Batch Size 8 Hands-on Time 1.00 0.25
Total Time 2.50 2.50
24 Hands-on Time 1.50 0.25
Total Time 3.00 2.50

96

No DNAse

Treatment

Hands-on Time NR 0.25
Total Time NR 2.00
96 Hands-on Time NR 0.25
Total Time NR 2.50

 

Products to Extract RNA from Tissue

PART # NAME PREPS
A32645 RNAdvance Tissue Kit  50
A32649 RNAdvance Tissue Kit  96
A32646 RNAdvance Tissue Kit  384

 

 

Not intended or validated for use in the diagnosis of disease or other conditions.
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